Sensitivity:

0.2ng/ml

Limit of   Detection:

0.8ng/ml

Assay   Performance:

Intra-assay CV% of 8.9; Inter-assay   CV% of 30

Contents:

Monoclonal antibody 1D8, Universal Allergen   Standard, Biotinylated antibody 7A1; sufficient reagents for 10 plates.

Storage:

Store at 4°C.

Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6
  Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
  Bovine Serum Albumin
  Streptavidin-Peroxidase
  1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Product Resources:

EL-D2   Certificate of Analysis

For research and commercial   use in vitro: not for human in vivo or therapeutic use.

Monoclonal Antibody:

1D8 (clone 1D8 C4 D8)

Vial:

Red Cap; 100μl

Immunogen:

Der p 2

Isotype:

Mouse IgG2A

Specificity:

Binds to a common epitope on mite Dermatophagoides spp. Group 2 allergens, Der p 2 and   Der f 2.

Purification:

From ascites by ammonium sulphate precipitation and purified   by affinity chromatography using Protein A. Single heavy and light chain   bands on SDS-PAGE.

Concentration:

1.35mg/ml in phosphate buffered saline, pH 7.4. Based on A280   for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

Certificate of Analysis

Monoclonal Antibody:

7A1 (clone 7A1 H1 G3)

Vial:

Brown Cap; 100μl

Immunogen:

Der f 2

Isotype:

Mouse IgG1

Specificity:

Binds to a common epitope on mite Dermatophagoides spp Group 2 allergens (Der f 2 and   Der p 2).

Purification:

Produced in ascites and purified by affinity chromatography   using Protein A. Single heavy and light chain bands on SDS-PAGE.

Biotinylation:

Biotinylated and titrated for use in ELISA at 1/1000 dilution.   Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative   free.

Certificate of Analysis

Allergen Standard:

Universal Allergen Standard

Vial:

White Cap; 400μl

Composition:

A formulation of eight purified natural allergens prepared in   1% BSA/50% glycerol/PBS, pH 7.4.

Concentration/Calibration:

Universal Allergen Standard

Protein Measurement

Concentration   (ng/ml)

Der p 1

Amino-acid analysis

2500

Der f 1

Amino-acid analysis

2500

Der p 2

Amino-acid analysis

1000

Fel d 1

Amino-acid analysis

1000

Can f 1

Amino-acid analysis

2500

Rat n 1

Amino-acid analysis

1000

Mus m 1

Amino-acid analysis

250

Bla g 2

Amino-acid analysis

2500

References:

1.  Heymann PW, Chapman MD, Aalberse RC, Fox JW,   Platts-Mills TAE.  Antigenic and structural analysis of group II   allergens (Der p II and Der f II) from house dust mites (Dermatophagoides   spp.).  J Allergy Clin Immunol 1989;83:1055-1067.

2.  Lombardero M, Heymann PW, Platts-Mills TAE, Fox JW,   Chapman MD.  Conformational stability of B cell epitopes on Group I   and Group II Dermatophagoides spp. allergens.  J Immunol   1990;144:1353-1360.

3.  DeBlay F, Heymann PW, Chapman MD, Platts-Mills   TAE.  Airborne dust mite allergens:  comparison of Group II   allergens with Group I mite allergen and cat allergen, Fel d I.  J   Allergy Clin Immunol 1991;88:919-926.

4.  Ovsyannikova IG, Vailes LD, Li Y, Heymann PW, Chapman   MD.  Monoclonal antibodies to Group II Dermatophagoides spp.   allergens:  Murine immune response, epitope analysis and development of   a two-site ELISA.  J Allergy Clin Immunol 1994;94:537-546.

5.  Platts-Mills TAE, Vervloet D, Thomas WR, Aalberse RC,   Chapman MD.  Indoor Allergens and Asthma: Report of the Third   International Workshop.  J Allergy Clin Immunol, 1997; 100:S1

(For complete reference list see EL-D2 Certificate of   Analysis)

Notes:

Product Resources:

EL-D2   Certificate of Analysis

For research and commercial   use in vitro: not for human in vivo or therapeutic use.