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Mite Group 2 ELISA kit (EL-D2)/尘螨过敏原

价    格:询价

产    地:美国更新时间:2019/7/15 13:41:34

品    牌:Indoor Biotechnologies型    号:kit

状    态:正常点击量:767

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上海博升生物科技有限公司

联 系 人:管小姐

电     话:021-54253791 / 400-670-5918

传     真:021-64280007

等     级: (第 10年)

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Mite Group 2 ELISA kit (EL-D2)/尘螨过敏原

Antibodies:

1D8/7A1

Sensitivity:

0.2ng/ml

Limit of   Detection:

0.8ng/ml

Assay   Performance:

Intra-assay CV% of 8.9; Inter-assay   CV% of 30

Contents:

Monoclonal antibody 1D8, Universal Allergen   Standard, Biotinylated antibody 7A1; sufficient reagents for 10 plates.

Storage:

Store at 4°C.

Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6
  Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
  Bovine Serum Albumin
  Streptavidin-Peroxidase
  1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:

 

Product Resources:

EL-D2   Certificate of Analysis

For research and commercial   use in vitro: not for human in vivo or therapeutic use.



产品参数

Mite Group 2 ELISA kit (EL-D2)/尘螨过敏原

Certificate of Analysis

 

Monoclonal Antibody:

1D8 (clone 1D8 C4 D8)

Vial:

Red Cap; 100μl

Immunogen:

Der p 2

Isotype:

Mouse IgG2A

Specificity:

Binds to a common epitope on mite Dermatophagoides spp. Group 2 allergens, Der p 2 and   Der f 2.

Purification:

From ascites by ammonium sulphate precipitation and purified   by affinity chromatography using Protein A. Single heavy and light chain   bands on SDS-PAGE.

Concentration:

1.35mg/ml in phosphate buffered saline, pH 7.4. Based on A280   for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

 
 

 

Certificate of Analysis

Monoclonal Antibody:

7A1 (clone 7A1 H1 G3)

Vial:

Brown Cap; 100μl

Immunogen:

Der f 2

Isotype:

Mouse IgG1

Specificity:

Binds to a common epitope on mite Dermatophagoides spp Group 2 allergens (Der f 2 and   Der p 2).

Purification:

Produced in ascites and purified by affinity chromatography   using Protein A. Single heavy and light chain bands on SDS-PAGE.

Biotinylation:

Biotinylated and titrated for use in ELISA at 1/1000 dilution.   Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative   free.

 
 

 

Certificate of Analysis

Allergen Standard:

Universal Allergen Standard

Vial:

White Cap; 400μl

Composition:

A formulation of eight purified natural allergens prepared in   1% BSA/50% glycerol/PBS, pH 7.4.

Concentration/Calibration:

 


Universal Allergen Standard

Protein Measurement

Concentration   (ng/ml)


Der p 1

Amino-acid analysis

2500


Der f 1

Amino-acid analysis

2500


Der p 2

Amino-acid analysis

1000


Fel d 1

Amino-acid analysis

1000


Can f 1

Amino-acid analysis

2500


Rat n 1

Amino-acid analysis

1000


Mus m 1

Amino-acid analysis

250


Bla g 2

Amino-acid analysis

2500


References:

1.  Heymann PW, Chapman MD, Aalberse RC, Fox JW,   Platts-Mills TAE.  Antigenic and structural analysis of group II   allergens (Der p II and Der f II) from house dust mites (Dermatophagoides   spp.).  J Allergy Clin Immunol 1989;83:1055-1067.

2.  Lombardero M, Heymann PW, Platts-Mills TAE, Fox JW,   Chapman MD.  Conformational stability of B cell epitopes on Group I   and Group II Dermatophagoides spp. allergens.  J Immunol   1990;144:1353-1360.

3.  DeBlay F, Heymann PW, Chapman MD, Platts-Mills   TAE.  Airborne dust mite allergens:  comparison of Group II   allergens with Group I mite allergen and cat allergen, Fel d I.  J   Allergy Clin Immunol 1991;88:919-926.

4.  Ovsyannikova IG, Vailes LD, Li Y, Heymann PW, Chapman   MD.  Monoclonal antibodies to Group II Dermatophagoides spp.   allergens:  Murine immune response, epitope analysis and development of   a two-site ELISA.  J Allergy Clin Immunol 1994;94:537-546.

5.  Platts-Mills TAE, Vervloet D, Thomas WR, Aalberse RC,   Chapman MD.  Indoor Allergens and Asthma: Report of the Third   International Workshop.  J Allergy Clin Immunol, 1997; 100:S1

(For complete reference list see EL-D2 Certificate of   Analysis)

Notes:

 

 

Product Resources:

EL-D2   Certificate of Analysis

 

For research and commercial   use in vitro: not for human in vivo or therapeutic use.

 



产品介绍

Mite Group 2 ELISA kit (EL-D2)/尘螨过敏原

INDOOR Biotechnologies Inc.(INDOOR)公司是一家专业开发生产用于研究哮喘以及其他过敏性疾病的免疫诊断和相关生物学检测产品的生物技术公司。公司在过敏原环境暴露水平的评估方面为世界领先。对过敏原结构,分子生物学和免疫反应的研究,公司处于世界前列。INDOOR公司致力于环境科学,过敏和哮喘相关的专业产品与服务,以供研究人员和消费者使用,通过研究、培训和开发尖端科技,为我们的客户和全球客户服务,以改善病人护理。INDOOR为政府及学术研究人员,全球500强的企业,室内空气质量的企业、过敏疫苗生产商和生物制药企业提供服务。 
       INDOOR生产的一系列过敏原研究和检测用抗体拥有来自美国弗吉尼亚大学的独家专利,覆盖范围广,包括屋尘螨、猫、狗、蟑螂、真菌和食物过敏原。这些抗体是包括ELISA和MARIA? 技术在内的过敏原检测和我们的Ventia? 快速过敏原检测实验分析平台的核心组分。IB的过敏原检测范围非常广泛,可用于对过敏原环境暴露水平的评估,也能让用户对使用的过敏原隔离清除程序、相关产品和设备的有效性进行可靠的评估,还可应用于过敏原标准化以及相关产品的研究开发。 
       INDOOR 还开发出了天然和重组的过敏原产品线,可用于研究和开发过敏原诊断产品和疫苗。这个产品线目前涵盖50多种纯化的过敏原,具有很高纯度和生物活性。这个产品线还包括低内毒素的纯化LoTox?过敏原,可用于过敏原和哮喘的免疫研究。 
       INDOOR的技术和产品已经在全球范围内被广泛使用,相关的科学论文已在超过250种科学出版物上发表。除提供产品以外IB还提供过敏原分析服务、合同研究服务以及专业培训服务。 



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