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4906837001-PhosSTOP™

价    格:2485

产    地:瑞士更新时间:2019/7/15 13:41:34

品    牌:罗氏型    号:4906837001

状    态:正常点击量:984

13788995069
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上海嵘崴达实业有限公司

联 系 人:runwelltac

电     话:021-64133189

传     真:021-64129208

等     级: (第 8年)

性     质:生产型,

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Features and Benefits

 Achieve immediate, effective, and convenient inhibition of a broad spectrum of phosphatases across a wide range of sample materials (e.g., mammalian, plant, yeast, and bacteria) with non-toxic PhosSTOP Phosphatase Inhibitor Cocktail Tablets. PhosSTOP is a proprietary blend of phosphatase inhibitors, formulated as a ready-to-use, quick-dissolving, water-soluble tablet.

Comprehensive protection: Inhibit acid and alkaline phosphatases, as well as serine/threonine (PP1, PP2A, and PP2B) and tyrosine protein phosphatases.
Safe: Dissolve the non-toxic PhosSTOP Tablets in aqueous solutions and avoid organic solvents (e.g., DMSO).
Suitable for formalin fixation: Maintain phosphorylation status of proteins in histological sections.
Convenient: Eliminate the time-consuming search for just the right phosphatase inhibitor.
Easy to use: Simply drop a quick-dissolving tablet into your buffer.
Stable: Store stock solutions for 1 month at +2 to +8°C, or for at least 6 months at -15 to -25°C.

For all-inclusive protein protection, combine PhosSTOP with cOmplete or cOmplete ULTRA Protease Inhibitor Tablets to inhibit both phosphatases and proteases.




产品参数

性质

                                                                   

          form   tablet
          packaging   pkg of 10 tablets (04906845001)
 pkg of 20 tablets (04906837001)
          mfr. no.   Roche
          solubility   aqueous buffer: soluble
          shipped in   wet ice
          storage temp.   2-8°C




产品介绍

 产品描述

PhosSTOP tablets are a proprietary blend of phosphatase inhibitors, formulated as a ready-to-use tablet which dissolves quickly in aqueous solutions (buffers), including buffers containing formalin (for formalin fixation).        

Working concentration: 1 tablet per 10 ml cell extract or buffer
Working solution: Solvent is recommended in distilled water or aqueous buffers (including buffers containing formalin).
Dissolve  one tablet per 10 ml aqueous buffer. Alternatively, prepare a 10x stock  solution in 1 ml dist. water or 100 mM phosphate buffer, pH 7.0. Higher  concentrations of stock solutions (e.g., 20x) are possible.
Storage conditions (working solution): 2 to 8 °C

Specificity

Protects proteins against dephosphorylation. Inhibits  phosphatase classes such as acid and alkaline phosphatases, as well as  serine/threonine (PP1, PP2A, and PP2B) and tyrosine protein phosphatases  (PTP) in bacterial, mammalian, yeast, and plant extracts.

Application

PhosSTOP? protects protein extracts against  dephosphorylation. The tablets inhibit a broad spectrum of phosphatases  such as acidic and alkaline phosphatases, as well as serine/threonine  (PP1, PP2A, and PP2B), dual-specificity phosphatases and tyrosine  protein phosphatases (PTP) in bacterial, insect, mammalian, yeast, and  plant extracts. PhosSTOP Tablets are well suited for the purification and detection of phoshorylated proteins.They are also used to prevent dephosphorylation in formalin-fixed paraffin embedded (FFPE) tissue sections.
PhosSTOP  tablets can be applied in combination with cOmplete Protease Inhibitor  Cocktail Tablets to simultaneously protect proteins against  dephosphorylation and proteolytic degradation. Both inhibitor tablets  maintain their activity when used together.
PhosSTOP inhibitors were  evaluated for their effect on protein assays. The inhibition shows  almost no influence on BCA or Bradford protein assays.




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