PhosSTOP tablets are a proprietary blend of phosphatase inhibitors, formulated as a ready-to-use tablet which dissolves quickly in aqueous solutions (buffers), including buffers containing formalin (for formalin fixation).        

Working concentration: 1 tablet per 10 ml cell extract or buffer
Working solution: Solvent is recommended in distilled water or aqueous buffers (including buffers containing formalin).
Dissolve  one tablet per 10 ml aqueous buffer. Alternatively, prepare a 10x stock  solution in 1 ml dist. water or 100 mM phosphate buffer, pH 7.0. Higher  concentrations of stock solutions (e.g., 20x) are possible.
Storage conditions (working solution): 2 to 8 °C

Specificity

Protects proteins against dephosphorylation. Inhibits  phosphatase classes such as acid and alkaline phosphatases, as well as  serine/threonine (PP1, PP2A, and PP2B) and tyrosine protein phosphatases  (PTP) in bacterial, mammalian, yeast, and plant extracts.

Application

PhosSTOP^? protects protein extracts against  dephosphorylation. The tablets inhibit a broad spectrum of phosphatases  such as acidic and alkaline phosphatases, as well as serine/threonine  (PP1, PP2A, and PP2B), dual-specificity phosphatases and tyrosine  protein phosphatases (PTP) in bacterial, insect, mammalian, yeast, and  plant extracts. PhosSTOP Tablets are well suited for the purification and detection of phoshorylated proteins.They are also used to prevent dephosphorylation in formalin-fixed paraffin embedded (FFPE) tissue sections.
PhosSTOP  tablets can be applied in combination with cOmplete Protease Inhibitor  Cocktail Tablets to simultaneously protect proteins against  dephosphorylation and proteolytic degradation. Both inhibitor tablets  maintain their activity when used together.
PhosSTOP inhibitors were  evaluated for their effect on protein assays. The inhibition shows  almost no influence on BCA or Bradford protein assays.