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现在位置首页>实验试剂制品>生化试剂>常规生化试剂> Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Pla

商品编号:67807
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Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Pla

价    格:5394

产    地:美国更新时间:2019/7/15 13:41:34

品    牌:R&D型    号:MLB00C

状    态:正常点击量:1011

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北京百奥创新科技有限公司

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现货特价促销,数量4,售完为止

Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate (1 KT)MLB00C

  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    4.8 pg/mL
  • Assay Range
    12.5 - 800 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse IL-1 beta
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.



产品参数

现货特价促销,数量4,售完为止

Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate (1 KT)MLB00C

Product Summary
The Quantikine Mouse IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 beta in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse IL-1 beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 beta.


Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma


Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020404040
Mean33.592.938634.693.5423
Standard Deviation2.54.311.72.96.223.9
CV%7.54.638.46.65.7


Recovery

The recovery of mouse IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.


Sample TypeAverage % RecoveryRange %
Cell Culture Supernates (n=4)10695-119
Cell Lysates (n=4)115110-120
EDTA Plasma (n=4)108101-117
Heparin Plasma (n=4)9787-114
Serum (n=4)10598-113
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-1 beta were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.


产品介绍

现货特价促销,数量4,售完为止

Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate (1 KT)MLB00C

  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    4.8 pg/mL
  • Assay Range
    12.5 - 800 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse IL-1 beta
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 beta in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse IL-1 beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 beta.


Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma


Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020404040
Mean33.592.938634.693.5423
Standard Deviation2.54.311.72.96.223.9
CV%7.54.638.46.65.7


Recovery

The recovery of mouse IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.


Sample TypeAverage % RecoveryRange %
Cell Culture Supernates (n=4)10695-119
Cell Lysates (n=4)115110-120
EDTA Plasma (n=4)108101-117
Heparin Plasma (n=4)9787-114
Serum (n=4)10598-113
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-1 beta were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 beta/IL-1F2
IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.
    • Long Name
      Interleukin 1 beta
    • Entrez Gene IDs
      3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig);
    • Alternate Names
      catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta;
      Related Research Areas
      • Cancer Biomarkers

      • Conventional/Classical Dendritic Cells

      • Cytokines and Receptors in Allergy and Asthma

      • Cytokines and Receptors in Angiogenesis

      • IL-1 Family

      • Infectious Diseases

      • Inflammatory Mediators

      • Isolation, Culture, and Identification of Natural Killer Cells

      • LTi Cells

      • Macrophage Activation Markers

      • Multiple Sclerosis and Myelin Related Molecules

      • Myeloid-derived Suppressor Cells (MDSC)

      • NCR-ILC3

      • Neuroinflammation

      • NOD-like Receptors and the Inflammasome

      • Platelets

      • Regulatory T Cells (Tregs)

      • T Cell Cytokine Signaling

      • Th17 Cells

      • Th9 Cells




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