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商品编号:52707
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Imidazole Buffer grade(咪唑)

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:A1073

状    态:正常点击量:2271

18210960361
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北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

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Imidazole Buffer grade

A1073

Order No. Quantity
A1073,0100 100 g
A1073,0500 500 g
A1073,1000 1 kg
A1073,5000 5 kg


产品参数

Synonym 1,3-Diaza-2,4-cyclopentadiene, Glyoxalin 
Solubility (20°C) 663 g/L (H2O) 
useful pH range 6.2 - 7.8 
pKa (20°C) 7.05 
Melting point 88 - 90°C 
Formula C3H4N2 
M 68.08 g/mol 
CAS-No. 288-32-4 
HS-No. 29332990 
EC-No. 206-019-2 
Storage RT 
LGK 8 B 
Disposal
R 22-34-63 
S 22-26-36/37/39-45 
Hazard-Symbol harmful, corrosive 
Class/PG 8/III 
UN-No. UN3263 
WGK
Specification  
Assay (titr.) min. 99 % 
Heavy metals (as Pb) max. 0.001 % 
Water max. 0.5 % 
Chloride max. 0.05 % 
Sulfate max. 0.05 % 


产品介绍

  (1)  Miles, E.W. (1977) Methods Enzymol. 47, 431-442
Modification of histidyl residues in proteins by diethylpyrocarbonate.
  (2)  Storrie, B. & Madden, E.A. (1990) Methods Enzymol. 182, 203-225
Isolation of subcellular organelles.
  (3)  Janknecht, R. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 8972-8976
Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.
  (4)  Ohkuma, Y. et al. (1995) Mol. Cell. Biol. 15, 4856-4866
Analysis of the role of TFIIE in basal transcription and TFIIH-mediated carboxy-terminal domain phosphorylation through structure-function studies of TFIIE-α.
  (5)  Marrakchi, N. et al. (1995) Biochim. Biophys. Acta 1244, 147-156
Cerastocytin, a new thrombin-like platelet activator from the venom of the tunisian viper Cerastes cerastes.
  (6)  Tavenier, M. et al. (1995) Biochim. Biophys. Acta< /i> 1244, 351-356
Kinetics of adenylate metabolism in human and rat myocardium.
  (7)  Eli, P. et al. (1999) J. Biochem. 125, 790-794
DNA denaturation with imidazole.

Hinweis

The high affinity of nickel-ions to histidine is suited very well for the purification of recombinant proteins, which are fused to a so-called ''His-tag'' (usually 6 histidine residues at the N- or C-terminus of the protein). This modified protein can be expressed in vaccinia infected cells (3), bacteria (4), yeast or insect cells (5) and purified by nickel-NTA-columns. The bound protein can be eluted by imidazole, which competes with the histidine residues of the his-tagged protein for the nickel ions. The optimal concentration of imidazole differs depending on the protein, but ranges very often from 40 to 200 mM.
Imidazole is often used as buffer substance for enzymatic reactions. The working concentration ranges e. g. from 20 mM (2) to 50 mM (5), but it can also reach 100 mM (6). A concentration of 1 M reduces the melting temperature of DNA by approx. 13°C and changes the mobility in the gel electrophoresis (7).
Caution: DEPC reacts with the non-protonated form of imidazol (1) and N-Carbethoxyimidazole will be formed. DEPC hydrolyses and it might be important to determine the exact DEPC concentration. This can be done by spectrophotometric measurements after incubation with 10 mM imidazole at 230 nm (pH 7.5).
Stability: Solutions of imidazole are stable for several years ( at least 6 years). It is not necessary to autoclave the solution and to store it protected from light. Buffers containing imidazole will turn to yellow. These solutions are still suitable to elute His-tag proteins.



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