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Formamide deionized(去离子甲酰胺 分子生物学级别)

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:A2156

状    态:正常点击量:2892

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北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

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Formamide deionized Molecular biology grade

A2156

Order No. Quantity
A2156,0100 100 ml
A2156,0250 250 ml
A2156,0500 500 ml
A2156,1000 1 L


产品参数

Synonym Formic acid amide 
Density (d 20°C/4°C) 1.13 
n 20°C/D 1.4472 
Melting point 2°C 
Boiling point 210°C 
Formula CH3NO 
M 45.04 g/mol 
CAS-No. 75-12-7 
HS-No. 29241900 
EC-No. 200-842-0 
Storage RT 
LGK 6.1 A 
Disposal
R 61 
S 53-45 
Hazard-Symbol toxic for reproduction 
WGK
Specification  
DNases/RNases/Proteases not detectable 
Assay (from N) min. 99.5 % 
Water max. 0.1 % 
Chloride max. 0.00005 % 
Fe max. 0.00001 % 
Pb max. 0.00001 % 


产品介绍

  (1)  Pinder, J.C. et al. (1974) Biochemistry 13, 5367-5373
Properties of RNA in Formamide.
  (2)  Pinder, J.C. et al. (1974) Biochemistry 13, 5373-5378
Electrophoresis of RNA in Formamide.
  (3)  Maniatis, T. & Efstratiadis, A (1980) Methods Enzymol. 65, 299-305
Fractionation of low molecular weight DNA or RNA on polyacrylamide gels containing 98 % Formamide or 7 M Urea.
  (4)  Meinkoth, J. & Wahl. G. (1984) Anal. Biochem. 138, 267-284
Hybridisation of nucleic acids, immobilized to membranes.
  (5)  Chomczynski, P. (1992) Nucleic Acids Res. 20, 3791-3792
Storage of RNA in formamide increases stability of the RNA.
  (6)  Cornish, E.C. et al. (1998) BioTechniques 25, 948-954
Improvement of the Southern blot method: Influence of the hybridisation buffer.
  (7)  Kafatos, F.C. et al. (1979) Nucleic Acids Res. 7, 1541-1552
Determination of nucleic acid sequence homologies with ''dot hybridization''.
  (8)  Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) 2001. Currrent Protocols in Molecular Biology. John Wiley & Sons, N.Y.

Hinweis

RNA and DNA with a chain length of >150 - 200 base pairs completely denature at room temperature in 98 % formamide, but not in 7 M urea. This allows the exact determination of the size of DNA or RNA single strands, because under these conditions the base composition and secondary structure have no influence on the migration behavior. The polyacrylamide gel contains 98 % deionized, anhydrous formamide. Acrylamide and bisacrylamide are dissolved in the formamide (1-4). The loading buffer contains formamide, too (see AppliChem''s loading buffers!). A similar technique of formamide-containing sequencing gels can be used for the sequencing of nucleic acids, when the secondary structure of the sequencing product causes an anormalous migration, i.e. compression of bands. Inclusion of up to 40 % of formamide is possible to overcome this problem (Ref. 8, pages 7.6.9-7.6.10 Supplement 16).
The stability of RNA is in formamide higher than in DEPC-treated water. RNA may be stored for more than a year in formamide at -20°C, instead of storage at -70°C in DEPC-treated water.
Besides gel electrophoresis deionized formamide is used for the hybridization of nucleic acids. Formamide reduces the melting temperature of a DNA-DNA hybrid by an average of 0.6°C per 1 % formamide; the maximum concentration is 50 %. By adding formamide, the temperature during the hybridization process can be reduced in comparison to an aqueous solution. At lower temperatures less DNA will detach from the nitrocellulose membrane. In addition, the background hybridization of heterologues RNA probes will be reduced. In combination with nylon membranes, no major advantage was observed.
A typical formamide-containing prehybridization/hybridization solution may be composed of: 5X SSC, 5X Denhardt solution, 50 % (w/v) formamide, 1 % (w/v) SDS and 100 μg/ml denatured salmon sperm DNA added just before use (ref. 8, p. 2.10.7 Supplement 35). The use of formamide-containing buffers increase the specific hybridisation in blotting experiments and reduces thereby the number of washing steps. This should be taken into account, if one switches to buffers without formamide (6).



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