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DTT BioChemica

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:A1101

状    态:正常点击量:1438

18210960361
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北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

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DTT BioChemica

A1101

Order No. Quantity
A1101,0005 5 g
A1101,0010 10 g
A1101,0025 25 g
A1101,0100 100 g


产品参数

Synonym Cleland''s Reagent, 1,4-Dithio-DL-threit(ol) 
Solubility (20°C) 1500 g/L (H2O) 
Melting point 42 - 44°C 
Formula C4H10O2S2 
M 154.25 g/mol 
CAS-No. 3483-12-3 
HS-No. 29309099 
EC-No. 222-468-7 
Storage 2-8°C 
LGK 10 - 13 
Disposal
R 22-36/38 
Hazard-Symbol harmful, irritant 
WGK 3* 
Specification  
Assay (iodometr.) min. 99.5 % 
pH (0.1 M; H2O; 20°C) 4.0 - 6.0 
DTT (oxidized) max. 0.5 % 
Water (K.F.) max. 0.5 % 
A (1 cm/0.02 M inH2O)  
283 nm max. 0.05 


产品介绍

 (1)  Cleland, W.W. (1964) Biochemistry 3, 480-482
Dithiothreitol, a new protective reagent for SH groups.
  (2)  Zahler, W.L. & Cleland, W.W. (1968) J. Biol. Chem. 243, 716-719
A specific and sensitive assay for disulfides.
  (3)  Jocelyn, P.C. (1987) Methods Enzymol. 143, 246-256
Chemical reduction of disulfides.
  (4)  Gegenheimer, P. (1990) Methods Enzymol. 182, 174-193
Preparation of plant extracts.
  (5)  Hart, R.A. et al. (1994) Bio/Technology 12, 1113-1117
''Large scale in situ isolation'' o periplasmatic IGF-I from E. coli.

Hinweis

Dithiothreitol (DTT) is, like β-mercaptoethanol, a reducing reagent for proteins and protects the cysteine residues against oxidation. It may substitute for β-mercaptoethanol in almost all experiments at three to four fold lower concentrations. DTT is less toxic, its odor is less intensive and it doesn''t form mixed disulfides like β-mercaptoethanol. DTT is water-soluble and stock solutions are prepared at 1 M. Store the solution aliquoted at -20°C and protect from heat during the experiment. Do not choose a to low concentration for the experiment, because it is readily oxidized by air. The working concentration ranges from 0.1 to 1 mM, but the preparation of plant extracts ( 5 mM; ref. 4) or for the ''large scale in situ isolation'' of proteins after fermentation (10 mM; ref. 5) require higher concentrations. For the complete reduction of disulfides, the concentration might be significantly higher (3).
A more stable and odorless alternative to DTT is Tris(2-carboxy)ethylphosphine (A2233).



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