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DAPI BioChemica

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:A1001

状    态:正常点击量:1544

18210960361
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北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

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DAPI BioChemica

A1001

Order No. Quantity
A1001,0010 10 mg
A1001,0025 25 mg
A1001,0100 100 mg


产品参数

Synonym 4'',6-Diamidino-2-phenylindole dihydrochloride 
Melting point approx. 330°C 
Formula C16H15N5 · 2HCl 
M 350.25 g/mol 
CAS-No. 28718-90-3 
HS-No. 29339980 
EC-No. 249-186-7 
Storage 2-8°C 
  protected from light 
LGK 10 - 13 
Specification  
Assay (TLC) min. 98 % 
Solubility (1 %; H2O) clear 
N min. 18 % 
UV spectrum  
λmax. 223 nm, 261 nm, 342 nm 
λmin. 246 nm, 282 nm 


产品介绍

 (1)  Russel, W.C. et al. (1975) Nature 253, 461-462
A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses.
  (2)  Kapuscinski, J. & Szer, W. (1979) Nucleic Acids Res. 6, 3519-3534
Interaction of 4'',6-diamidine-2-phenylindole with synthetic polynucleotides.
  (3)  Wilson, W.D. et al. (1989) J. Am. Chem. Soc. 111, 5008-5010
Binding of 4'',6-Diamidino-2-phenylindole (DAPI) to GC and mixed sequences in DNA: Intercalation of a classical groove-binding molecule.
  (4)  Otto, F. (1990) Methods Cell Biol. 33, 105-110
DAPI-staining of cells fixed for "flow cytometry".
  (5)  Schweizer, D. (1976) Chromosoma 58, 307-324
''Reverse Fluorescent Chromosome Banding'' with Chromomycin and DAPI.
  (6)  Naimski, P. et al. (1980) Anal. Biochem. 106, 471-475
Quantitative Fluoreszenzanalyse verschiedener Konformationen von DAPI-gebundener DNA.
  (7)  Daxhelet, G.A. et al. (1989) Anal. Biochem. 179, 401-403
Spectrofluorometry of Dyes with DNAs of Different Base Composition and Conformation.
  (8)  Xia, H. et al. (1997) BioTechniques 22, 934-936
Detection of Mycoplasma infections of mammalian cells.

Hinweis

DAPI is an excellent dye for the staining of DNA. Originally, only the specific binding to AT-base pairs without an intercalation was known (2), but later on, the intercalation into GC-base pairs was shown (3). The most popular application of DAPI is its use as a reagent to detect mycoplasma or virus DNA (e. g. vaccinia infection or ''unwanted'' viral contamination of cell culture cells) in the cell culture.
AppliChem recommends the following simple procedure: Grow cells on a coverslip in a cell culture dish to reach approx. 70 % confluence. Pour off the medium from the cells. Wash the coverslip once with 1 μg/ml DAPI in methanol. Incubate the cells on the coverslip at 37°C for 15 minutes in 1 μg DAPI/ml in methanol. Pour off the staining solution and wash the coverslip once with methanol. Put it up-side-down on a slide with PBS or glycerol as mounting medium. Do not use water. Examine the cells under a microscope (excitation: 365 nm; emission maximum at 450 nm). Prolonged incubation with DAPI increases the nuclear fluorescence, shorter incubation time leads to a weaker nuclear staining, which facilitates the examination of the cytoplasmic fluorescence.
Solubility / Stability: Dissolve DAPI in double-distilled water to a final concentration of 1 - 5 mg. The maximum solubility in water is approx. 25 mg/ml. DAPI is insoluble in PBS. Do not use any buffers. Dilute the stock solution with methanol to a final concentration of 1 μg/ml. Solutions are stable at room temperature for 1 - 2 weeks (4), at +4°C up to 6 months and frozen between 6 and 12 months (1 ml aliquots). If the solution becomes turbid, DAPI is hydrolyzed. DAPI bleaches quickly in contact with light, even if it is quite stable against UV-light. Incubate your samples in the dark. If your samples are stored at +4°C for one day, fluorescence is stabilzed.



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