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Bromophenol blue sodium salt Electrophoresis grade

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:a3640

状    态:正常点击量:1410

18210960361
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北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

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Bromophenol blue sodium salt Electrophoresis grade(溴酚蓝钠盐)

A3640

Order No. Quantity
A3640,0005 5 g
A3640,0010 10 g
A3640,0025 25 g


产品参数

Synonym 3’,3’’,5’,5’’-Tetrabromophenolsulfone-phthalein sodium salt 
Formula C19H9Br4NaO5
M 691.94 g/mol 
CAS-No. 34725-61-6 
HS-No. 29349990 
EC-No. 252-170-2 
Storage RT 
LGK 10 - 13 
Specification  
Assay (photometr.) min. 90 % 
λmax. (pH 4.6) 590 - 595 nm 
Transition interval complies 
Loss on drying max. 5 % 


产品介绍

(1)  Maxam, A.M. & Gilbert. W. (1977) Proc. Natl. Acad. Sci. USA 74, 560-564
A new method for sequencing DNA.
  (2)  Ogden, R.C. & Adams. D.A. (1987) Methods Enzymol. 152, 61-87
Electrophoresis in agarose and acrylamide gels.
  (2)  Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  (4)  Cathey, J.C. et al. (1997) BioTechniques 22, 222
Incorporation of bromophenol blue enhances visibility of polyacrylamide gels.
  (5)  Shieh, B. et al. (1998) BioTechniques 24, 60-64
Stain-induced denaturation of water-solubilized DNA.
  (6)  Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) 2000. Currrent Protocols in Molecular Biology. page 7.6.5 Suppl.
  16 John Wiley & Sons, New York.

Hinweis

Bromophenol blue (BPB) and xylene cyanol FF (XC) are the most widely used stains in gel electrophoresis for tracing the migration of samples on electrophoretic gels. Usually, both stains are added to the sample buffers (loading buffers; see our ready-to-use loading buffers) at concentrations of 0.05 % to 0.25 %. In the vertical polyacrylamide gel electrophoresis it may happen, that the gel is leaking or air bubbles may come up. If the gel is staind with 1 - 5 mg bromophenol blue, this may be detected much easier and earlier. In case that the stain will interfere with other applications (e. g. silver staining), a pre-run of the gel will remove bromophenol blue. It does not influence the migration of the samples. Add the solid stain before adding the catalyst (4). If DNA is dissolved in bidistilled water (pH 5.5 - 6.0) - not in TE buffer - both stains, as well bromophenol blue as xylene cyanol FF may change the migration rate during the electrophoresis run. The bands run faster than expected (5).
In denaturing gels, the dye migrates with sizes like the following oligonucleotide:
Polyacrylamide (%) Bromophenol blue Xylene cyanol (according to ref. 1, 4)
5 35 bases 130 bases
6 26 bases 106 bases
8 19 bases 75 bases
10 12 bases 55 bases
20 10 bases 28 bases



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