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商品编号:52652
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Tetracycline hydrochloride(四环素盐酸盐)

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:A2228

状    态:正常点击量:1865

18210960361
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北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

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Tetracycline hydrochloride

A2228

Order No. Quantity
A2228,0025 25 g
A2228,0050 50 g
A2228,0100 100 g


产品参数

Synonym Achromycine hydrochloride 
Melting point 215 - 220°C 
Formula C22H24N2O8 · HCl 
M 480.90 g/mol 
CAS-No. 64-75-5 
HS-No. 29413000 
EC-No. 200-593-8 
Storage RT 
  protected from light 
LGK 10 - 13 
Specification  
Assay (titr.) min. 95 % 
α20°C/D; 1%, 0.01 M HCl -239° bis -250° 
pH (1 %; H2O) 2.0 - 3.0 
Sulfated ash max. 0.5 % 
Loss on drying max. 2 % 


产品介绍

 (1)  Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  (2)  Gossen, M. & Bujard, H. (1992) Proc. Natl. Acad. Sci. USA 89, 5547-5551
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
  (3)  Gossen, M. et al. (1995) Science 268, 1766-1769
Transcriptional activation by Tetracyclines in mammalian cells.
  (4)  Yin, D.X. et al. (1996) Anal. Biochem. 235, 195-201
Tetracycline-controlled gene expression system achieves high-level and quantitative control of gene expression.
  (5)  Freundlieb, S. et al. (1997) Methods Enzymol. 283, 159-173
Tetracyclin-controlled gene expression systems for investigations of the cell cycle.

Hinweis

Tetracycline was prepared first syntheticaly from chlorotetracycline, later it was isolated from Streptomyces viridifaciens. Tetracycline is active against gram positive and gram negative cocci and bacteria. The introduction of the tetracycline-controlled gene expression system brought a new application for the antibiotic molecular biology (2-4).
Stability: Tetracycline hydrochloride is stable if stored in a dry place and protected from light. It is water-soluble with approx. 11 mg/ml at 28°C. The stability in solutions is optimal at pH 3 - 5.2 (shelf life approx. 6 - 12 days). It may be stored between -20°C and 37°C. Since Tetracyclin is light-sensitive, solutions and agar plates containing TC should be protected from light. The recommended working concentration is 10 - 50 μg/ml, the stock solution e.g. 1.25 mg/ml. Magnesium ions antagonize with the activity of TC.



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