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Tetracycline hydrochloride（四环素盐酸盐）
Tetracycline hydrochloride（四环素盐酸盐）
价 格：询价
产 地：德国更新时间：2021/9/10 14:45:14
品 牌：AppliChem型 号：A2228
状 态：正常点击量：2580
联 系 人：方丽初
电 话：010-88597838
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Tetracycline hydrochloride
A2228
| Order No. |
Quantity |
| A2228,0025 |
25 |
g |
| A2228,0050 |
50 |
g |
| A2228,0100 |
100 |
g |
产品参数
| Synonym |
Achromycine hydrochloride |
| Melting point |
215 - 220°C |
| Formula |
C22H24N2O8 · HCl |
| M |
480.90 g/mol |
| CAS-No. |
64-75-5 |
| HS-No. |
29413000 |
| EC-No. |
200-593-8 |
| Storage |
RT |
| |
protected from light |
| LGK |
10 - 13 |
| Specification |
|
| Assay (titr.) |
min. 95 % |
| α20°C/D; 1%, 0.01 M HCl |
-239° bis -250° |
| pH (1 %; H2O) |
2.0 - 3.0 |
| Sulfated ash |
max. 0.5 % |
| Loss on drying |
max. 2 % |
产品介绍
(1) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
(2) Gossen, M. & Bujard, H. (1992) Proc. Natl. Acad. Sci. USA 89, 5547-5551
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
(3) Gossen, M. et al. (1995) Science 268, 1766-1769
Transcriptional activation by Tetracyclines in mammalian cells.
(4) Yin, D.X. et al. (1996) Anal. Biochem. 235, 195-201
Tetracycline-controlled gene expression system achieves high-level and quantitative control of gene expression.
(5) Freundlieb, S. et al. (1997) Methods Enzymol. 283, 159-173
Tetracyclin-controlled gene expression systems for investigations of the cell cycle.
Tetracycline was prepared first syntheticaly from chlorotetracycline, later it was isolated from Streptomyces viridifaciens. Tetracycline is active against gram positive and gram negative cocci and bacteria. The introduction of the tetracycline-controlled gene expression system brought a new application for the antibiotic molecular biology (2-4).
Stability: Tetracycline hydrochloride is stable if stored in a dry place and protected from light. It is water-soluble with approx. 11 mg/ml at 28°C. The stability in solutions is optimal at pH 3 - 5.2 (shelf life approx. 6 - 12 days). It may be stored between -20°C and 37°C. Since Tetracyclin is light-sensitive, solutions and agar plates containing TC should be protected from light. The recommended working concentration is 10 - 50 μg/ml, the stock solution e.g. 1.25 mg/ml. Magnesium ions antagonize with the activity of TC.
