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商品编号:52651
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Puromycin dihydrochloride BioChemica(嘌呤霉素盐酸盐)

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:A2856

状    态:正常点击量:1882

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北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

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Puromycin dihydrochloride BioChemica(嘌呤霉素盐酸盐)

Order No. Quantity
A2856,0005 5 mg
A2856,0010 10 mg
A2856,0025 25 mg
A2856,0100 100 mg


产品参数

Synonym Purimycin 
Formula C22H29N7O5 · 2HCl 
M 544.44 g/mol 
CAS-No. 58-58-2 
HS-No. 29419000 
EC-No. 200-387-8 
Storage 2-8°C 
LGK 10 - 13 
Specification  
Assay (HPLC) min. 98 % 
IR-Spectrum complies 
Solubility (5 %; H2O) clear, colorless 


产品介绍

(1)  Sambrook, J. & Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition Page 16.49 + 17.65. CSHL Press, Cold Spring Harbor, NY.
  (2)  De La Luna, S. & Ortin, J. (1992) Methods Enzymol. 216, 376-385
pac-Gen as Efficient Dominant Marker and Reporter Gene in Mammalian Cells.

Hinweis

Puromycin is an aminonucleoside antibiotic from Streptomyces alboniger. It is an analogon to aminoacyl-tRNA and inhibits the protein synthesis by termination of the peptidyl transfer at the ribosomes in prokaryotes and eukaryotes. The antibiotic inhibits the growth of gram positive bacteria and different animal cells. Fungi and gram negative bacterias are resistant, since puromycin cannot pass the cell wall. The discovery of the puromycin-N-acetyltransferase (PAC) in a Streptomyces-stem allowed to employ the antibiotic as a selection marker, comparable to neomycin. The working concentration is for mammalian cells depending on the cell line between 1 and 50 μg/ml.
Stock solutions of puromycin (10 mg/ml) can be prepared in HEPES buffer and are stored at +4°C or -20°C (improves stability) up to one year.



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