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Pepstatin A(抑肽素)

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:A2205

状    态:正常点击量:1909

18210960361
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北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

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Pepstatin A

Order No. Quantity
A2205,0010 10 mg
A2205,0025 25 mg
A2205,0100 100 mg


产品参数

Synonym Isovaleryl-L-val-L-val-statinyl-L-ala-statine 
Formula C34H63N5O9 
M 685.91 g/mol 
CAS-No. 26305-03-3 
HS-No. 29241900 
EC-No. 247-600-0 
Storage 2-8°C 
LGK 10 - 13 
Specification  
Assay (HPLC) min. 98 % 


产品介绍

(1)  Aoyagi, T. et al. (1971) J. Antibiotics 24, 687-694
Effect of Pepstatin on acid proteases.
  (2)  Umezawa, H. (1976) Methods Enzymol. 45, 678-695
Structures and activities of protease inhibitors of microbial origin.
  (3)  Katoh, I. et al. (1987) Nature 329, 654-656
Inhibition of retroviral protease activity by an aspartyl proteinase inhibitor.
  (4)  Von der Helm, K. et al. (1989) FEBS Lett. 247, 349-352
Inhibition of HIV replication in cell culture by the specific aspartic protease inhibitor Pepstatin A.
  (5)  Menéndez-Arias, L. et al. (1992) J. Biol. Chem. 267, 24134-24139
Purification and characterization of the MMTV protease expressed in Escherichia coli.
  (6)  Saiga, A. et al. (1993) Arch. Virol. 128, 195-210
The HTLV-1 protease expressed in Escherichia coli possesses aspartic proteinase activity.

Hinweis

Pepstatin was isolated from Streptomyces testaceus and different actinomycetes. It is a strong inhibitor of acidic proteases (''Aspartic Proteases''): Pepsin, HIV and MMTV protease, cathepsin D and renin (1-6). Pepstatin is not a strong toxin (1, 2). The working concentration ranges from 1 μM (0.7 μg/ml) to 5 μM, whereas the MMTV protease requires a higher dose (IC50 = 90 μM, ref. 5). Solutions are stable for approx. 1 week at +4°C or 1 month at -20°C.
Pepstatin may be dissolved in methanol or ethanol at a concentration of 1 mg/ml.. It is of low solubility in ethyl acetate, ether, benzene, chloroform, acetic acid, DMSO, pyridine and water (2). Under the assay conditions described in reference 3 (concentration 1 μM - 1 mM in DMSO), a precipitate of pepstatin A was observed.



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