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DEPC BioChemica

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:A0881

状    态:正常点击量:1760

18210960361
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北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

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DEPC BioChemica

A0881

Order No. Quantity
A0881,0020 20 ml
A0881,0050 50 ml
A0881,0100 100 ml
A0881,0250 250 ml


产品参数

Synonym Diethyldicarbonate, Diethylpyrocarbonate 
Density (d 20°/4°) 1.122 
n 20°/D 1.398 
Boiling point 160 - 163°C 
Formula C6H10O5 
M 162.14 g/mol 
CAS-No. 1609-47-8 
HS-No. 29209085 
EC-No. 216-542-8 
Storage 2-8°C 
LGK 3 B 
Disposal
R 20/22-36/37/38 
S 26-36 
Hazard-Symbol harmful, irritant 
Specification  
Assay (NT) min. 97 % 


产品介绍

(1)  Summers, W.C. (1970) Anal. Biochem. 33, 459-463
A simple method for the extraction of RNA from E. coli with DEPC.
  (2)  Berger, S.L. (1975) Anal. Biochem. 67, 428-437
DEPC: An examination of its properties in buffered solutions with a new assay technique.
  (3)  Ehrenberg, L. et al. (1976) Progr. Nucl. Acid Res. Mol. Biol. 16, 189-262
Diethyl pyrocarbonate in nucleic acid research.
  (4)  Miles, E.W. (1977) Methods Enzymol. 47, 431-442
Modification of Histidyl residues in proteins by Diethyl pyrocarbonate.
  (5)  Herr, W. (1985) Proc. Natl. Acad. Sci. USA 82, 8009-8013
DEPC: A chemical probe for secondary structure in negatively supercoiled DNA.
  (6)  McCarthy, J.G. et al. (1990) Biochemistry 29, 6071-6081
Chemical reactivity of DEPC with B-DNA: Specific reactivity with short A-Tracts.

Hinweis

Diethylpyrocarbonate (DEPC) modifies histidyl-residues in proteins and leads to the inactivation of many enzymes. In molecular biology it is mainly used as a strong, but not absolute, inhibitor of RNase activity. In addition, DEPC reacts with adenosine of single-stranded nucleic acids (2) and purines in Z-DNA (4) and B-DNA (5), respectively. For the treatment of water with DEPC, add 1 ml of DEPC to 1 liter bidistilled water (0.1 % DEPC v/v) and stirr over night. Autoclaving at 20 psi for 20 minutes inactivates DEPC. DEPC reacts with water and hydrolyses to ethanol and CO2. It is of low solubility in aqueous buffers (solubility in water max. 40 mM). DEPC in its pure form has a concentration of 6.9 M (3). As a diluent you may use anhydrous ethanol or acetonitrile (e. g. stock solution 10 mM, ref. 3). Autoclaving totaly inactivates DEPC in solutions, even if the typical smell does not disappear (1). Since DEPC acylates histidyl- and tyrosyl-residues, do not use it in combination with reagents containing these residues (e. g. Tris). Tris inactivates DEPC!
Caution: DEPC is suspected to be a carcinogen and should be handled with great care. Do not breathe fumes and avoid contact with skin! DEPC may damage eyes and mucous membranes! The combination with ammonia ions (e.g. ammonium acetate from the precipitation of nucleic acids) forms the strong carcinogen ethylcarbamate (2).



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