来宝网Logo

热门词:生物显微镜 水质分析仪 微波消解 荧光定量PCR 电化学工作站 生物安全柜

现在位置首页>实验试剂制品>生化试剂>常规生化试剂> Aprotinin BioChemica(蛋白酶抑制剂)

商品编号:52625
分享

Aprotinin BioChemica(蛋白酶抑制剂)

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:A2132

状    态:正常点击量:1830

18210960361
联系我时,请说明是在来宝网上看到的,谢谢!

北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

联系我时请说在来宝网上看到的,谢谢!




Aprotinin BioChemica

A2132

Order No. Quantity
A2132,0010 10 mg
A2132,0025 25 mg
A2132,0100 100 mg


产品参数

Synonym Trasylol® 
origin from bovine lung 
Formula C284H432N84O79S7 
M 6511.52 g/mol 
CAS-No. 9087-70-1 
HS-No. 35040090 
Storage 2-8°C 
LGK 10 - 13 
WGK
  ® registered trademark of Bayer AG 
Specification  
Assay (protein) min. 90 % 
Activity (KIU/mg) min. 6000 
pH (1 %; H2O) 5.0 - 7.5 
Water (K.F.) max. 6 % 


产品介绍

 (1)  Kassell, B. & Laskowski, Sr., M. (1965) Biochem. Biophys. Res. Com. 20, 463-468.
The basic Trypsin inhibitor of bovine pancreas: V. The disulfide linkages.
  (2)  Kassell, B. (1970) Methods Enzymol. 19, 844-852
Bovine Trypsin-Kallikrein inhibitor (Kunitz Inhibitor, Basic Pancreatic Trypsin Inhibitor, Polyvalent Inhibitor from Bovine Organs).
  (3)  Fritz, H. & Wunderer, G. (1983) Drug Res. 33, 479-494
Biochemistry and application of Aprotinin, the Kallikrein inhibitor from bovine organs.

Hinweis

Aprotinin was isolated independently from two laboratories and originally named Bovine Pancreatic Trypsin Inhibitor (BPTI) and Trypsin-kallikrein Inhibitor (TKI). It is a strongly basic protein (58 amino acids; ref. 1, 2). Aprotinin inhibits trypsin, chymotrypsin, kallikrein from different sources and plasmin. Its recommended working concentration is 2 - 10 μg/ml (approx. 1 μM). The working concentration given in ref. 3 (500 KIU/ml; approx. 10 μM) seems to be very high.
Stability: Aprotinin may be stored indefinite at +4°C in lyophilized form. Dissolved in saline or buffer solutions (e. g. 10 mg/ml) at pH 5 - 8, it may be stored aliquoted at +4°C at least for one month or frozen at -20°C for several years. Thawed aliquots of the stock solutions should not be frozen again, because repeated freeze-thawing will lead to aggregation of the polypeptide aprotinin. Solutions with a pH value below 4 or above 9 should be used immediately. Protect from direct sunlight, UV light and reducing agents. It is stable in water, 70 % methanol, 70 % ethanol or 50 % acetone, too (2, 3).
Unit-Definition: One Trypsin Inhibitor Unit (TIU) will decrease the activity of 2 trypsin units by 50 %, where one trypsin unit will hydrolyze 1.0 μmole Nα-benzoyl-DL-arginine p-nitroanilide (BAPNA) per minute at pH 7.8 and 25°C. 1 TIU (Trypsin Inhibitor Unit) corresponds to approx. 1300 KIU (Kallikrein Inhibitor Units) and 1 TIU corresponds to one Ph. Eur. Unit.



猜你喜欢