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Urea BioChemica尿素

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:A1360

状    态:正常点击量:1266

18210960361
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北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

联系我时请说在来宝网上看到的,谢谢!




Order No. Quantity
A1360,1000 1 kg
A1360,5000 5 kg
A1360,9010 10 kg


产品参数

Synonym Carbamide 
Solubility (20°C) 1000 g/L (H2O) 
Melting point 132 - 135°C 
Formula CH4N2
M 60.06 g/mol 
CAS-No. 57-13-6 
HS-No. 31021010 
EC-No. 2003155 
Storage RT 
LGK 10 - 13 
WGK
Specification  
Assay (from N) min. 99 % 
Heavy metals (as Pb) max. 0.001 % 
Water max. 0.5 % 
Chloride max. 0.005 % 
Sulfate max. 0.005 % 
Fe max. 0.001 % 
A (1 cm/8 M in water)  
260 nm max. 0.05 
280 nm max. 0.02 


产品介绍

(1)  Maniatis, T. & Efstratiadis, A (1980) Methods Enzymol. 65, 299-305
Fractionation of low molecular weight DNA or RNA in polyacrylamide gels containing 98 % formamide or 7 M urea.
  (2)  Soulié, S. et al. (1996) Anal. Biochem. 236, 363-364
Urea reduces the aggregation of membrane proteins during SDS-PAGE.
  (3)  Lippincott, J. & Apostol. I. (1999) Anal. Biochem. 267, 57-64
Carbamylation of cysteine in hemoglobin by urea.

 

Hinweis

RNA or DNA denature at 7 M urea in polyacrylamide gels. Molecules with chain lengths larger than 150 - 200 nucleotides do not completely denature at room temperature in 7 M urea, so it is necessary to use polyacrylamide gels containing 98 % formamide to size larger molecules.
Separation of proteins according to their size and charge requires a concentration of 6-8 M urea in the probe and in the stacking and separation gel. At alkaline pH values urea decomposes and forms cyanate ions. These ions may react with the amino group and form stable carbamylated derivatives with a changed migration pattern during electrophoresis. Solutions containing urea should not be stored for longer periods, should not be heated and gels containing urea should prerun to remove cyanate ions before loading the gel.



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