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现在位置首页>实验试剂制品>生化试剂>常规生化试剂> Coomassie® Brilliant blue R-250 (C.I. 42660)考马斯亮蓝

商品编号:52537
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Coomassie® Brilliant blue R-250 (C.I. 42660)考马斯亮蓝

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:AppliChem型    号:A1092

状    态:正常点击量:3077

18210960361
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北京西美杰科技有限公司

联 系 人:方丽初

电     话:010-88597838

传     真:010-88595011

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

联系我时请说在来宝网上看到的,谢谢!




Order No. Quantity
A1092,0010 10 g
A1092,0025 25 g
A1092,0100 100 g


产品参数

Synonym Brilliant blue R, Xylenebrilliantcyanine 
Formula C45H44N3NaO7S2 
M 825.98 g/mol 
CAS-No. 6104-59-2 
HS-No. 32041200 
EC-No. 2280605 
Storage RT 
LGK 10 - 13 
WGK
  ® registered trademark of Imperial Industries PLC 
Specification  
λmax. (buffer pH 7.0) 554 - 563 nm 
E 1 %,1 cm, λmax. >300 (pH 7.0) 


产品介绍

(1)  Fazekas De St. Groth, S. et al. (1963) Biochim. Biophys. Acta 71, 377-391
Two new staining procedures for quantitative estimation of proteins on electrophoresis strips.
  (2)  Chrambach, A. et al. (1967) Anal. Biochem. 20, 150-154
A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.
  (3)  Neuhoff, V. et al. (1988) Electrophoresis 9, 255-262
Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie® Briliant Blue G-250 and R-250.
  (4)  Choi, J.-K. et al. (1996) Anal. Biochem. 236, 82-84
Modified Coomassie® Blue staining of proteins in polyacrylamide gels with Bismark brown.
  (5)  Tal, M. et al. (1985) J. Biol. Chem. 260, 9976-9980
Why does Coomassie® Brilliant Blue R Interact Differently with Different Porteins?

Coomassie® Brilliant Blue R-250 is one of the most commonly used stains for proteins, after their separation by polyacrylamide gel electrophoresis. The protein-dye complex has an absorption maximum at 549 nm, the dye without protein at 555 nm (in 0.01 M citrate buffer, pH 3). The intensity in staining of proteins probably depends on the basicity of a protein (5). Per positively charged amino acid approximately 1.5 - 3 molecules of Coomassie® will be bound. This variation complicate the exact protein determination with albumin as a standard, since this protein contains more basic amino acids than many other proteins (5).
There do exist many protocols for sensitive staining procedures with Coomassie® (e. g. ref. 3, 4). The sensitivity reaches a limit at 25 ng protein (4). We recommend the following protocol:
I. Staining solution: 0.1 % Coomassie® Brilliant Blue R-250 (Prod.-No. A1092)
20 % methanol (or ethanol)
10 % acetic acid
The SDS gel (without ''''stacking gel'''') is stained for 1 hour at 60°C or for 2 hours at 50°C or over night at RT.
II. Destaining solution: 20 % methanol (or ethanol)
10 % acetic acid
Destain the gel for 3 - 4 hours at 50 - 60°C. Add some sponges.
Subsequently wash the gel for 15 minuts in water and dry under vacuum at 60°C for 2 - 3 hours



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