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德国Stratec公司PSP Spin Stool DNA Plus Kit(1038110300)!乐博生物,优势订购!

北京乐博生物科技有限公司2016年8月27日 18:44 点击:1636

德国Stratec公司PSP Spin Stool DNA Plus Kit(1038110300)!乐博生物,优势订购!
 

英文名称:PSP Spin Stool DNA Plus Kit
中文名称:粪便DNA提取试剂盒
品牌:Stratec
产地:德国
货号:1038110300
保存温度:15-30°C

 

北京乐博生物联系方式:
邮箱:info@lab-bio.com
网站: www.lab-bio.com


The PSP Spin Stool DNA Plus Kit is an integrated system for collection, transportation and storage of stool samples and subsequent DNA purification. Transportation of the stabilized DNA can be carried out in the DNA Stabilizer solution without refrigeration at ambient temperature. The purification kit is designed for DNA isolation from pathogenic microorganisms and from the host organism.

Benefits

    All-in-one product - from collection to isolation: pre-filled collection tubes with Stool DNA stabilizer
    Samples are stable at room temperature for 3 months for easy transport and storage
    Inactivation of DNases and preservation of the microorganism titre
    For In Vitro Diagnostic Use (CE-IVD)*
    Product characteristics

    Starting material
        

    Average yield
        

    Preparation time

    a spoon of stool sample
    (about 1 g) collected in
    8 ml of Stool Stabilizer
        

    up to 50 μg/ 1.4 ml of
    stabilized stool sample
        

    about 60 min

    Workflow

    For the DNA extraction process a small aliquot of the total volume is used, and the residual sample can be stored in the DNA stabilization buffer at -20°C for further extractions. The PSP Spin Stool DNA Plus Kit uses a precleaning step, optimized buffer and essential washing conditions to remove potent inhibitors very efficiently. The purified DNA is ready to use for subsequent downstream applications:


    • PCR applications
    • hybridization
    • genetic typing
    • pathogen typing
    • mutation analysis
    • sequencing
    • ancestry research
    • feed and food determination


    *) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

    Ordering information

    Product
        

    Package Size
        

    Catalogue No

    PSP Spin Stool DNA Plus Kit
        

    50 purifications
        

    1038110200

    PSP Spin Stool DNA Plus Kit
        

    250 purifications
        

    1038110300

    Stool Collection Tubes
    with Stool DNA Stabilizer
        

    50 prefilled tubes
        

    1038111200

    Stool Collection Tubes
    with Stool DNA Stabilizer
        

    250 tubes
        

    1038111300

    Stool DNA Stabilizer
        

    250 ml
        

    1038111100
    1. High DNA yields from stool samples

    Total DNA was isolated using the PSP Spin Stool DNA Plus Kit and a competitor kit Q from 20 different stool samples according to the standard protocols. The total DNA concentration was measured on a NanoDrop (figure A) and the respective microorganism DNA content (from Bifidobacterium sp., Escherichia coli, Faecalibacterium prausnitzii, Lactobacillus sp.) was analyzed and measured via quantitative real-time PCR (figure B).

    Data kindly provided by S.C. Bischoff, University Hohenheim, Stuttgart, Germany

    2. Detection of Helicobacter pylori in human stool samples


    Amplification of different target sequences of extracted bacterial DNA (H. pylori) using the PSP Spin Stool DNA Plus Kit from human stool samples stored at room temperature (without refrigeration) for 3 days with Stool Stabilizer in a Stool Collection Tube.

    3. Detection of Mycobacterium avium ssp. paratuberculosis in stool samples from cattle


    DNA was isolated from clinical and spiked stool samples from cattle using the PSP Spin Stool DNA Plus Kit. The isolated DNA was used for PCR amplification of the M. avium ssp. paratuberculosis target sequence ISMav2 (using the Bactotype Amplification Kit Mycobacterium paratuberculosis) with a sensitivitiy of 102 cells/g stool (6-fold repeat).

    Data kindly provided by Dr. F. Kloep, Biotype AG.
    User manual download

    PSP Spin Stool DNA/ Plus Kit
    MSDS download

    Stool Collection Tubes  with Stool DNA Stabilizer

    InviAdsorb

    Zirconia Beads II

    Binding Buffer A

    Proteinase K

    Wash Buffer I

    Wash Buffer II

    Elution Buffer
    Selected references

    A comparison of rumen microbial profiles in dairy cows as retrieved by 454 Roche and Ion Torrent (PGM) sequencing platforms.
    Indugu N, Bittinger K, Kumar S, Vecchiarelli B, Pitta D
    PeerJ. 2016 Feb 4;4:e1599
    An Integrated Metabolomic and Microbiome Analysis Identified Specific Gut Microbiota Associated with Fecal Cholesterol and Coprostanol in Clostridium difficile Infection.
    Antharam VC, McEwen DC, Garrett TJ, Dossey AT, Li EC, Kozlov AN, Mesbah Z, Wang GP
    PLoS One. 2016 Feb 12;11(2):e0148824
    Bidirectional interactions between indomethacin and the murine intestinal microbiota.
    Liang X, Bittinger K, Li X, Abernethy DR, Bushman FD, FitzGerald GA
    Elife. 2015 Dec 23;4:e08973
    The stool microbiota of insulin resistant women with recent gestational diabetes, a high risk group for type 2 diabetes.
    Fugmann M, Breier M, Rottenkolber M, Banning F, Ferrari U, Sacco V, Grallert H, Parhofer KG, Seissler J, Clavel T, Lechner A
    Sci Rep. 2015 Aug 17;5:13212
    Rhythmicity of the intestinal microbiota is regulated by gender and the host circadian clock.
    Liang X, Bushman FD, FitzGerald GA
    Proc Natl Acad Sci U S A. 2015 Aug 18;112(33):10479-84
    Effects of surgical and dietary weight loss therapy for obesity on gut microbiota composition and nutrient absorption.
    Damms-Machado A, Mitra S, Schollenberger AE, Kramer KM, Meile T, Königsrainer A, Huson DH, Bischoff SC
    Biomed Res Int. 2015;2015:806248
    Seeking the source of Pseudomonas aeruginosa infections in a recently opened hospital: an observational study using whole-genome sequencing.
    Quick J, Cumley N, Wearn CM, Niebel M, Constantinidou C, Thomas CM, Pallen MJ, Moiemen NS, Bamford A, Oppenheim B, Loman NJ
    BMJ Open. 2014 Nov 4;4(11):e006278
    Temporal dynamics in the ruminal microbiome of dairy cows during the transition period.
    Pitta DW, Kumar S, Vecchiarelli B, Shirley DJ, Bittinger K, Baker LD, Ferguson JD, Thomsen N
    J Anim Sci. 2014 Sep;92(9):4014-22
    Reagent contamination can critically impact sequence-based microbiome analyses.
    Susannah Salter, Michael J Cox, Elena M Turek, Szymon T Calus, William O Cookson, Miriam F Moffatt, Paul Turner, Julian Parkhill, Nick Loman, Alan W Walker
    doi: http://dx.doi.org/10.1101/007187
    Fecal microbial composition of ulcerative colitis and Crohn's disease patients in remission and subsequent exacerbation.
    Wills ES, Jonkers DM, Savelkoul PH, Masclee AA, Pierik MJ, Penders J
    PLoS One. 2014 Mar 7;9(3):e90981
    Life-threatening angioedema of the tongue: the detection of the RNA of B henselae in the saliva of a male patient and his dog as well as of the DNA of three Bartonella species in the blood of the patient.
    Lösch B, Wank R
    BMJ Case Rep. 2014 Mar 20;2014
    Analysis of the intestinal microbiota using SOLiD 16S rRNA gene sequencing and SOLiD shotgun sequencing.
    Mitra S, Förster-Fromme K, Damms-Machado A, Scheurenbrand T, Biskup S, Huson DH, Bischoff SC
    BMC Genomics. 2013;14 Suppl 5:S16
    Chronic kidney disease alters intestinal microbial flora.
    Vaziri ND, Wong J, Pahl M, Piceno YM, Yuan J, DeSantis TZ, Ni Z, Nguyen TH, Andersen GL
    Kidney Int. 2013 Feb;83(2):308-15
    Differentiation of Entamoeba histolytica/Entamoeba dispar by the polymerase chain reaction in stool samples of patients with gastrointestinal symptoms in the Sanliurfa Province.
    Zeyrek FY, Turgay N, Unver A, Ustün S, Akarca U, Töz S
    Turkiye Parazitol Derg. 2013;37(3):174-8
    Structural modulation of gut microbiota in life-long calorie-restricted mice.
    Zhang C, Li S, Yang L, Huang P, Li W, Wang S, Zhao G, Zhang M, Pang X, Yan Z, Liu Y, Zhao L
    Nat Commun. 2013;4:2163
    A sensitive and specific multiplex PCR approach for sex identification of ursine and tremarctine bears suitable for non-invasive samples.
    Bidon T, Frosch C, Eiken HG, Kutschera VE, Hagen SB, Aarnes SG, Fain SR, Janke A, Hailer F
    Mol Ecol Resour. 2013 May;13(3):362-8
    Succession in the gut microbiome following antibiotic and antibody therapies for Clostridium difficile.
    Peterfreund GL, Vandivier LE, Sinha R, Marozsan AJ, Olson WC, Zhu J, Bushman FD
    PLoS One. 2012;7(10):e46966
    A tool kit for quantifying eukaryotic rRNA gene sequences from human microbiome samples.
    Dollive S, Peterfreund GL, Sherrill-Mix S, Bittinger K, Sinha R, Hoffmann C, Nabel CS, Hill DA, Artis D, Bachman MA, Custers-Allen R, Grunberg S, Wu GD, Lewis JD, Bushman FD
    Genome Biol. 2012 Jul 3;13(7):R60. [Epub ahead of print]
    Limited gene flow among brown bear populations in far Northern Europe? Genetic analysis of the east-west border population in the Pasvik Valley.
    Schregel J, Kopatz A, Hagen SB, Brøseth H, Smith ME, Wikan S, Wartiainen I, Aspholm PE, Aspi J, Swenson JE, Makarova O, Polikarpova N, Schneider M, Knappskog PM, Ruokonen M, Kojola I, Tirronen KF, Danilov PI, Eiken HG
    Mol Ecol. 2012 Jul;21(14):3474-88
    Innate lymphoid cells promote anatomical containment of lymphoid-resident commensal bacteria.
    Sonnenberg GF, Monticelli LA, Alenghat T, Fung TC, Hutnick NA, Kunisawa J, Shibata N, Grunberg S, Sinha R, Zahm AM, Tardif MR, Sathaliyawala T, Kubota M, Farber DL, Collman RG, Shaked A, Fouser LA, Weiner DB, Tessier PA, Friedman JR, Kiyono H, Bushman FD, Chang KM, Artis D
    Science. 2012 Jun 8;336(6086):1321-5
    The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosus [corrected] coprodetection.
    Salant H, Abbasi I, Hamburger J
    Am J Trop Med Hyg. 2012 Nov;87(5):883-7
    Sampling and pyrosequencing methods for characterizing bacterial communities in the human gut using 16S sequence tags.
    Wu GD, Lewis JD, Hoffmann C, Chen YY, Knight R, Bittinger K, Hwang J, Chen J, Berkowsky R, Nessel L, Li H, Bushman FD
    BMC Microbiol. 2010 Jul 30;10:206

 

北京乐博生物联系方式:
邮箱:info@lab-bio.com
网站: www.lab-bio.com
(来源: 北京乐博生物科技有限公司


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